The hero of the series, Fone Bone is the most courageous of the Bones but also the youngest. He and his cousin Smiley Bone help their other cousin Phoney Bone escape from Boneville after he upset the villagers, and get stuck in the Valley. He is the most level-headed and the smartest of the three Bone cousins.
The tallest of the Bones, the middle cousin and arguably the least intelligent, he plays a one-string instrument. Smiley is often seen smoking a cigar, and often irritates characters with his simple-mindedness, even when seeking to help people.
Manipulative and greedy, Phoney Bone is the eldest and least courageous of the Bones and he will do anything to get rich. Run out of Boneville by an angry mob of villagers after trying to run for mayor of Boneville, his greediness and selfishness make an enemy of anyone who crosses him in the Valley.
The son of the great dragon Mim, The Great Red Dragon is often Fone Bone's last-minute savior. The Red Dragon appears when he is most needed. Gran'ma Ben does not trust him, regardless of how many times he has saved her or her friends from harm. The Great Red Dragon seems to be incredibly ancient. In a sequence that shows the land during the Dragons' reign, supposedly the beginning of time, the Great Red Dragon can be seen fighting Mim along with other dragons.
A helpful Acanalonia bivittata, or planthopper, who appears as a recurring supporting character. Often mistaken for a leaf, Ted is the first creature Fone Bone encounters when he enters the valley and the two become fast friends. He harbors a strange link to the Red Dragon and has an older brother who is several hundred times his size. He is able to perform magical enchantments and has the ability to detect Ghost Circles.
Ted's big brother is Ted's older brother, the second creature Fone Bone meets in the Valley. He is always there to clean up a problem with Ted, especially if his younger brother is mistaken for a leaf. Not much is known about Ted's older brother, but in Ted's words, "He's actual a real nice guy!"
Two rat creature soldiers, one blue, one brown, who have a particular interest in devouring the Bone cousins, and Fone Bone in particular. The two are rather incompetent, once deserting the army after their disobedience costs Kingdok his arm and later allying with the Bones briefly before returning to their own side. They address each other as "comrade".